PHYSIOLOGICAL AND BLOOD BIOCHEMICAL RESPONSES OF Pangasianodon hypophthalmus ENRICHED PELLETS Citrus jambhiri L.
DOI:
https://doi.org/10.31258/ajoas.8.3.327-336Keywords:
Jungga lime, Pangasionodon hypopthalmus, Blood Biochemical, MAS diseaseAbstract
Jungga lime (Citrus jambhiri L.)/Sundai acid/Limau kuit is a natural ingredient that contains alkaloids, saponins, steroids, tannins, flavonoids, ascorbic acid, essential oils, and limonene, which are antibacterial. This research was conducted from August to December 2024 at the Laboratory of Fish Diseases and Parasites, Faculty of Fisheries and Marine, Universitas Riau. This research aimed to analyze the physiological and biochemical responses of the blood of striped catfish. The method used was experimental with a completely randomized design (CRD) of 1 factor, five treatments, and three replicates. The treatments applied were Kn (without the addition of jungga lime and without being infected with A. hydrophila), Kp (without jungga lime and infected with A. hydrophila), and jungga lime-enriched pellets at P1 (6.0 mL/kg), P2 (6.5 mL/kg), and P3 (7.0 mL/kg), and infected with A. hydrophila. Striped catfish (P. hypophthalmus) with a size of 8-12 cm and a weight of 6-7 g were kept in a 54x37x29 cm container, with a stocking density of 1 fish/3 L, and fed 3 times a day and sanitation. On day 32, fish were intramuscularly challenged with A. hydrophila (108 CFU/mL) at 0.1 mL. The results showed that the best dose was P3 (7.0 mL/kg) with a blood glucose of 79.33 mg/dL, Mg 3.07 mg/dL, Ca 9.87 mg/dL, P 4.03 mg/dl, Lisozyme activity of 259 units, mL, weight growth reached 19.11 g, and length growth reached 5.39 cm. Adding jungga lime to fish feed can improve post-challenge fish blood's physiological and biochemical responses
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1. Handayani, R., Rejeki, S. Evaluasi Kelayakan Usaha Budidaya Ikan Bandeng (Chanos chanos) Secara Semi Intensif di Kecamatan Ulujami, Kabupaten Pemalang. Jurnal Sains Akuakultur, 2019; 3(1): 9-16
2. Anwar, M.T.E., Tugiyono, T. Isolasi dan Identifikasi Bakteri Aeromonas hydrophila pada Ikan Lele Dumbo (Clarias gariepinus). Jurnal Pertanian Agros, 2023; 25(3): 2073-2078
3. Astriani, N.K., Chusniasih, D., Marcellia, S. Uji Aktivitas Antibakteri Ekstrak Daun Jeruk Purut (Citrus hystrix) terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Ilmu Kedokteran dan Kesehatan, 2021; 8(3): 291-301
4. Sembiring, H.B. Aktivitas Antibakteri dan Antioksidan Minyak Atsiri Daun Asam Jungga (Citrus jambhiri Lush.). Chimica et Natura Acta, 2018; 6(1): 19-24.
5. Sihombing, J.M.A. Sensitivitas Perasan Buah Jeruk Purut (Citrus hystrix) terhadap Bakteri Aeromonas hydrophila. Universitas Riau. Pekanbaru, 2019.
6. Artati, D., Oman, M. Identifikasi Bakteri Aeromonas hydrophila Menggunakan Kit Api 20E di Laboratorium Mikrobiologi BRPI Sukamandi. Buletin Teknik Litkayasa Akuakultur, 2020; 18(1): 75-80
7. Ginting, K.D., Riauwaty, M., Syawal, H. Diferensiasi Leukosit Ikan Lele Dumbo (Clarias gariepinus) yang diberi Pakan Mengandung Kunyit (Curcuma domestica Val.) dan Diinfeksi Bakteri Aeromonas hydrophila. Jurnal Ilmu Perairan (Aquatic Science), 2021; 9(2): 116-125
8. Pattanayak, S., Priyadarsini, S., Paul, A., Kumar, P.R., Sahoo, P.K. Diversity of Virulence-Associated Genes in Patogenic Aeromonas hydrophila Isolates and Their in Vivo Modulation at Varied Water Temperatures. Microbial Patogenesis, 2020; 147(104424)
9. Farag, M.R., Alagawany, M., Taha, H.S., Ismail, T.A., Khalil, S.R., Abou-Zeid, S.M. Immune Response and Susceptibility of Nile Tilapia Fish to Aeromonas hydrophila Infection Following the Exposure to Bifenthrin and/or Supplementation with Petroselinum crispum Essential Oil. Ecotoxicology and Environmental Safety, 2021; 216(112205): 1-9
10. Mohammed, A.M.H., Ibrahim, M.A., Omran, A.A., Mohamed, E.M., Elsheikh, S.E. Minerals Content, Essential Oils Composition and Physicochemical Properties of Citrus jambhiri Lush. (Rough Lemon) from the Sudan. International Letters of Chemistry, Physics and Astronomy, 2013; 9(1): 25-30
11. Ariyanti, I., Marnani, S., Setiawan, A.C., Syakuri, H., Dadiono, M.S. Profil Darah Ikan Nila (Oreochromis niloticus) yang Diberi Pakan dengan Penambahan Ekstrak Daun Mangrove Api-Api Putih (Avicennia marina). Jurnal Perikanan Pantura, 2022; 5(2): 215-226
12. Addini, N., Tang, U.M., Syawal, H. Fisiologis Pertumbuhan Ikan Selais (Ompok hypophthalmus) pada Sistem Resirkulasi Akuakultur (SRA). Berkala Perikanan Terubuk, 2020; 48(2): 1-14
13. Johann, S., Oliveira, V.L.D., Pizzolatti, M.G., Schripsema, J., Braz-Filho, R., Branco, A., Smânia Jr, A. Antimicrobial Activity of Wax and Hexane Extracts from Citrus spp. Peels. Memórias do Instituto Oswaldo Cruz, 2007; 102(6): 681-685
14. Hamdan, D.I., Abdulla, R.H., Mohamed, M.E., ElShazly, A.M. Chemical Composition and Biological Activity of Essential Oils of Cleopatra Mandarin (Citrus reshni) Cultivated in Egypt. Journal of Pharmacognosy and Phytotherapy, 2013; 5(5): 83-90.
15. Bojarski, B.M., Socha, E.D., Kozak, A.R., Bryzek, S., Kanipos, L., Szala, E., Kondera, K., Lugowska, M., Witeska. Does the Site of Blood Collection in Fish Affect Haematological and Blood Biochemical Results. Folia Biologica (Krakow), 2021; 69(2): 51-56
16. Putra, R. Gambaran Darah dan Aspek Fisiologis Ikan Mas (Cyprinus carpio) yang Diberi Pakan Mengandung Jamu Fermentasi dan Dipelihara pada Salinitas Berbeda. Program Pasca Sarjana Universitas Riau. Pekanbaru, 2023.
17. Sharma, M., Kaur, V.I., Ansal, M.D. Physiological Responses of Freshwater Ornamental Fish Koi Carp, Cyprinus carpio (L.) in Inland Saline Water: Growth and Hematological Changes. Indian Journal of Ecology, 2017; 44(4): 864–868
18. Syawal, H., Lukistyowati, I., Putra, R. Hematological and Physiological Characteristics of the Common Carp (Cyprinus carpio) Reared in Saline Water and Fed with Fermented Herbs. Aquaculture, Aquarium, Conservation & Legislation, 2023; 16(1): 299-306
19. Kubılay, A., Uluköy, G. The Effects of Acute Stress on Rainbow Trout (Oncorhynchus mykiss). Turkish Journal of Zoology, 2002; 26(2): 249-254
20. Fuadi, N., Dewiyanti, I., Hasri, I. Pengaruh Pemberian Probiotik dan Vitamin C dalam Pakan Komersil terhadap Pertumbuhan Ikan Depik (Rasbora tawarensis). Jurnal Ilmiah Mahasiswa Kelautan Perikanan Unsyiah, 2019; 4(3)
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Copyright (c) 2025 Cindy Naomi Fadilla Christine Simalango, Morina Riauwaty, Henni Syawal (Author)
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